PROGRAMME
22nd of October
Pre-Symposium
08:30
: Registration in the Main Auditorium
09:00 - 09:30: Welcome by the Organizer
09:30 - 10:30: Introductory
Lecture of the ‘Chair of Honor’ - Video Conference
-
.........................Hans
Lambers
..........The
role and control of the alternative oxidase in higher plants:
a century of research on cyanide-resistant respiration.
10:30 - 11:00: coffee break
Pre-Symposium
Sessions
Chair: Miquel Ribas-Carbo
11:00 - 12:00: Mark G. Tjoelker
.....................Plant
respiration and temperature: acclimation and adaptation in a warming
world.
12:00 - 14:00: Lunch
14:00 - 15:00: Lee D. Hansen
.....................Calorespirometic
determination of growth rates and substrate carbon conversion
efficiencies of plant tissues.
15:00 - 15:30: coffee break
15:30 - 16:30: Ko Noguchi
.....................AOX
and light intensity.
17:00 - 19:00: Small Buffet for all participants
23rd
of October
Main
Symposium
Opening Session & Introductory Lecture
starting at 10:00 am
Welcome
and
Opening of the Symposium
Themes:
Provocation: Life is a fantastic risk –
why not deciding for a scientific career?
Hypothesis:
Accepting the risk of leading-edge research benefits the socio-economy!
Jorge Araújo
Rector of the University of Évora, Portugal
João Sentieiro
President of the Foundation for Science and Technology, FCT, Portugal
Raffaele Liberali
Director of the Energy Program at DG Research, European Commission
former Director for ‘The Human Factor, Mobility and Marie
Curie activities’, DG Research, Belgium
Benvindo Maçãs
Coordinator of the Genetic Resourses, Ecophysiology and Plant
Breeding Unit
INRB/L-INIA, Elvas, Portugal
Introductory Lecture:
Birgit Arnholdt-Schmitt
EU Marie Curie Chair, University of Évora, Portugal
‘AOX – Research: From fundamental knowledge
to application’
12:30
- 14:30: Lunch
SESSION I. AOX Protein Research
...Hypothesis:
...Revealing
the structure of AOX is making progress!
...The number of functional
sites in AOX genes is higher than expected!
...Chair:
Deborah Berthold
15:00 - 15:30: Allison McDonald
.....................The taxonomic
distribution of alternative oxidase across all kingdoms of life:
what information can sequence comparisons give us?
15:30 - 16:00: Mary Albury
.....................Biochemical
and structural studies of the alternative oxidase.
16:00 - 16:30: coffee break
16:30 - 17:00: Deborah Berthold
.....................A
family resemblance? Characterization of Coq7, a distant cousin
of the alternative oxidase.
17:00 - 17:30: Round-table discussion
18:30: Reception in the
City Hall
24th
of October
SESSION
II. AOX DNA Research
cHypothesis:
..Organisation
of genomic AOX sequences and gene expression patterns are highly
variable!
...Chairs:
Alexios N. Polidoros and Hélia C. Cardoso
09:00 - 09:30: Alexios
N. Polidoros
.....................Aox
gene structure, transcript variation and expression in plants.
09:30 - 10:10: Diego Breviario
.....................Stimulating
AOX-DNA research and functional marker development through the
foreseeable exploitation of the AOX genome loci features: the
example is set by the small nuclear gene family of plant tubulin.
10:10 - 10:40: coffee break
10:40 - 10:55: Alexandre Ferreira
.....................Intron
polymorphisms in an alternative oxidase gene (AOX1b) from different
Hypericum perforatum L. genotypes
10:55 - 11:10: Doroteia Campos
.....................The
alternative oxidase gene family from Daucus carota L. reveals
a new gene expansion pattern and polymorphic sequences
11:10 - 11:25: Moisés Xavier and Birgit
Arnholdt-Schmitt
.....................Computational
prediction of S/MARs in the vicinity of alternative oxidase genes
11:25 - 12:00: Poster Session
............'Poster
Flashes'
............-
Vitis vinifera alternative oxidase family: An attractive model
for genomic design.
............-
AOX
gene sequences as potential functional marker candidates for efficient
adventitious root induction in Olea europea L
............-
Regulatory pathways controlling gene expression of stress responsive
mitochondrial proteins in Arabidopsis.
............-
AOX genes isolation in stone pine (Pinus pinea L.)
.............Discussion
at the posters
12:00 -12:30: Round-table discussion
....................Chair:
Birgit Arnholdt-Schmitt
....................Alexios
Polidoros
....................Diego
Breviário
....................Hélia
Cardoso
....................Olivier
Van Aken
12:30 - 14:00: Lunch
SESSION III. AOX: Molecular-Physiology
and Stress Research
...Hypothesis:
...AOX
can play a critical role in cell reprogramming under stress!
...Chair:
Allan Rasmusson
14:15
- 15:00: Miquel Ribas-Carbo
.....................When
is AOX actually activated?
15:00 - 15:20: Kollipara Padmasree
.....................Relative
importance of AOX pathway over COX pathway in optimizing photosynthesis
under light, osmotic or temperature stress.
15:20 - 15:40: Olivier Van Aken
.....................Mitochondrial
and chloroplast retrograde regulation converge via abscisic acid
signalling factors.
15:40
- 16:00: Ana Rita Matos
.....................Up-regulation
of alternative oxidase in Arabidopsis lipid mutants under low
temperature.
16:00 - 16:30: coffee break
16:30 - 17:15: Allan Rasmusson
.....................Expressional
control of alternative respiratory pathways in response to changes
in the plant environment.
18:00: Visit to Fundação Eugénio
de Almeida
25th
of October
SESSION
III. (CONTINUATION)
...Chair:
Miquel Ribas-Carbo
09:00 - 09:45: Greg Vanlerberghe
.....................Does
alternative oxidase act as a master regulator of a mitochondrial-
based stress signaling pathway?
09:45 - 10:15: coffee break
10:15 - 12:30: Poster session
'Poster Flashes'
- Alternative oxidase
(AOX) pathway in Arabidopsis thaliana leaves with altered levels
of AOX protein under well-watered and water stress conditions.
-
Importance of COX and AOX pathways in optimizing photosynthesis
under light, osmotic or temperature stress: role of ROS and antioxidants.
Salicylic acid induces alterations on respiration and membrane
lipid composition of soybean mitochondria.
- A GmAOX2b antisense gene
is linked to repressed reproductive success in soybean.
-
Aox and stress in cucumber MSC16 mitochondrial mutant.
- The overexpression of
alternative oxidase protects the plant under salinity stress.
- Characterization of mutants
of the alternative respiratory pathway of Arabodopsis thaliana.
-
Alternative oxidase genes can be transiently suppressed
during local lesion formation in different plant-virus interactions.
-
The role of AOX and pyruvate in controling the plant internal
oxygen concentration.
-
Studies on the AOX involvement in cell reprogramming -
Part I - Suppression of the ability to realize somatic embryogenesis
by an AOX inhibitor in embryogenic carrot cells
-
Studies on the AOX involvement in cell reprogramming -
Part II - AOX expression levels during carrot somatic embryogenesis
............Discussion
at the posters
12:30 - 14:15: Lunch
...Research
beyond the plant level
...Chair:
João D. Arrabaça
14:30 - 15:15: Frank Nargang
.....................Identification
of genes controlling alternative oxidase expression in the filamentous
fungus Neurospora crassa
15:15 - 15:25: 'Poster
Flashes'
........-
Metabolic
changes in citric acid-producing Aspergillus niger by disruption
and overexpression of alternative oxidase gene.
........-
Physiological
role of alternative oxidase in the yeast Candida maltosa.
15:25 - 16:00: coffee break
16:00 - 16:30: Pierre Rustin
.....................An
international consortium to promote the use of alternative respiratory
chain proteins as tools to better understand and counteract respiratory
chain deficiencies.
16:30 - 16:50: Marek Babusiak
.....................Aox
in Drosophila- a new tool for understanding and therapy of mitochondrial
disorders.
16:50 – 17:00: 'Poster
Flashes'
......-
Alternative oxidase compensate cytochrome oxidase deficiency consequences
in human cells.
......-
Mitochondrial cyanide-resistant respiratory pathway alleviates
generation of reactive oxygen species by complex III inhibitor,
antimycin A, in human cells.
17:00 - 18:00: Discussion
at the posters
18:00
- 19:00: Round-table discussion
.....................Chair:
Greg Vanlerberghe
.....................Allan
Rasmusson
.....................Miquel
Ribas-Carbo
.....................Ko
Noguchi
.....................Lee
Hansen
.....................Frank
Nargang
.....................Pierre
Rustin
26th
of October
SESSION
IV. Genetics and Plant Breeding
...Hypothesis:
...AOX
sequence polymorphism can assist Molecular Plant Breeding!
...Chairs:
Birgit Arnholdt-Schmitt and Oliver Gailing
09:00
- 09:45: Oliver Gailing
.....................Genetic
and genomic approaches to assess adaptative genetic variation
In plants: Forest trees as a model.
09:45 - 10:15: Kathleen Soole
.....................Manipulation
of alternative oxidase as a mechanism for salt tolerance.
10:15 - 10:45: coffee break
10:45 - 11:05: Hélia Cardoso
.....................Insertion/deletion
and single nucleotide polymorphisms in AOX2 genes of Daucus carota
L.
11:05 - 11:30: Poster Session
.........'Poster
Flashes'
.........-
DNA polymorphism studies in partial Aox1d and Aox2 sequences from
Olea europaea L. - A potential contribution for functional marker
development.
.........-
The AOX1a gene of Daucus carota L. demonstrates diverse polymorphisms
between genotypes.
.........-
Can AOX be applied as functional marker in AMF communities?
.........Discussion
at the posters
11:30 - 12:00: Round-table discussion
12:00 - 13:30: Lunch
13:30: Excursion
to the National Station of Plant Breeding in Elvas, visit to the
‘Coffee Museum’ and ‘Cellar Mayor’.
19:30: Symposium dinner in a restaurant in Campo
Maior.
27th
of October
SESSION V. Reports Main
Sessions in reference to the Hypotheses and Final Paper Preparation
10:00
- 12:00: I. Parallel Session Meetings including all Group
Representatives
.....................A.
Session I Report
.....................B.
Session II Report
.....................C.
Session III Report
.....................D.
Session IV Report
12:00 - 14:00: Lunch
14:00 - 15:30: II. Final Paper Preparation
.......................Consortium
of all participants
15:30 - 16:00: coffee break
16:00 - 17:00: Concluding Session
